如何进行超像素图像分割和特征提取

问题描述

我对皮肤组织的多类分割感兴趣,我将 3000 个皮肤组织标签分为 4 类,我创建了一个 CNN 分类算法来训练我的分类模型。我想将分类模型用于新皮肤组织图像的分割任务,并对属于每个类的皮肤组织进行特征提取

以下是用于训练我的分类模型的代码

from tensorflow.keras.layers import Input,Concatenate,Dropout,Flatten,Dense,GlobalAveragePooling2D,Conv2D
from tensorflow.keras import backend as K
#from tensorflow.keras.utils import np_utils
from tensorflow.keras.utils import to_categorical
from tensorflow.keras.preprocessing.image import ImageDataGenerator
from tensorflow.keras import optimizers
from tensorflow.keras.metrics import top_k_categorical_accuracy
from tensorflow.keras.models import Sequential,Model,load_model
import tensorflow as tf
from tensorflow.keras.initializers import he_uniform
from tensorflow.keras.callbacks import ModelCheckpoint,LearningRateScheduler,TensorBoard,EarlyStopping,CSVLogger,ReduceLROnPlateau
#from tensorflow.compat.keras.backend import KTF
#import keras.backend.tensorflow_backend as KTF
from tensorflow.keras.applications.resnet50 import resnet50
from tensorflow.keras.applications.inception_v3 import InceptionV3

import os
import matplotlib.pylab as plt
import numpy as np
import pandas as pd
#import numpy as np,Pillow,skimage,imageio,matplotlib
#from scipy.misc import imresize
from skimage.transform import resize
from tqdm import tqdm
from tensorflow.keras import metrics

#### PREPROCESS STAGE ####

# Path to superpixels class files
classes_file = "/home/DEV/SKIN_3000_CLASSES.csv"
concatenated_data= pd.read_csv(classes_file,header=None)

# Instances with targets
targets = concatenated_data[1].tolist()

# Split data according to their classes
class_0 = concatenated_data[concatenated_data[1] == 0]
class_1 = concatenated_data[concatenated_data[1] == 1]
class_2 = concatenated_data[concatenated_data[1] == 2]
class_3 = concatenated_data[concatenated_data[1] == 3]

# Holdout split train/test set (Other options are k-folds or leave-one-out)
split_proportion = 0.8

split_size_0 = int(len(class_0)*split_proportion)
split_size_1 = int(len(class_1)*split_proportion)
split_size_2 = int(len(class_2)*split_proportion)
split_size_3 = int(len(class_3)*split_proportion)

new_class_0_train = np.random.choice(len(class_0),split_size_0,replace=False)
new_class_0_train = class_0.iloc[new_class_0_train]
new_class_0_test = ~class_0.iloc[:][0].isin(new_class_0_train.iloc[:][0])
new_class_0_test = class_0[new_class_0_test]

new_class_1_train = np.random.choice(len(class_1),split_size_1,replace=False)
new_class_1_train = class_1.iloc[new_class_1_train]
new_class_1_test = ~class_1.iloc[:][0].isin(new_class_1_train.iloc[:][0])
new_class_1_test = class_1[new_class_1_test]

new_class_2_train = np.random.choice(len(class_2),split_size_2,replace=False)
new_class_2_train = class_2.iloc[new_class_2_train]
new_class_2_test = ~class_2.iloc[:][0].isin(new_class_2_train.iloc[:][0])
new_class_2_test = class_2[new_class_2_test]

new_class_3_train = np.random.choice(len(class_3),split_size_3,replace=False)
new_class_3_train = class_3.iloc[new_class_3_train]
new_class_3_test = ~class_3.iloc[:][0].isin(new_class_3_train.iloc[:][0])
new_class_3_test = class_3[new_class_3_test]

x_train_list = pd.concat(
    [new_class_0_train,new_class_1_train,new_class_2_train,new_class_3_train])
x_test_list = pd.concat(
    [new_class_0_test,new_class_1_test,new_class_2_test,new_class_3_test])

# Load superpixels files
imagePath = "/home/DEV/SKIN_SET_3000/"

x_train = []
y_train = []
for index,row in tqdm(x_train_list.iterrows(),total=x_train_list.shape[0]):
    try:
        loadedImage = plt.imread(imagePath + str(row[0]) + ".jpg")
        x_train.append(loadedImage)
        y_train.append(row[1])
    except:
        # Try with .png file format if images are not properly loaded
        try:
            loadedImage = plt.imread(imagePath + str(row[0]) + ".png")
            x_train.append(loadedImage)
            y_train.append(row[1])
        except:
            # Print file names whenever it is impossible to load image files
            print(imagePath + str(row[0]))

x_test = []
y_test = []
for index,row in tqdm(x_test_list.iterrows(),total=x_test_list.shape[0]):
    try:
        loadedImage = plt.imread(imagePath + str(row[0]) + ".jpg")
        x_test.append(loadedImage)
        y_test.append(row[1])
    except:
        # Try with .png file format if images are not properly loaded
        try:
            loadedImage = plt.imread(imagePath + str(row[0]) + ".png")
            x_test.append(loadedImage)
            y_test.append(row[1])
        except:
            # Print file names whenever it is impossible to load image files
            print(imagePath + str(row[0]))


# Reescaling of images 
img_width,img_height = 139,139

index = 0
for image in tqdm(x_train):
    #aux = resize(image,(img_width,img_height,3),"bilinear")
    aux = resize(image,img_height))
    x_train[index] = aux / 255.0  # normalization
    index += 1

index = 0
for image in tqdm(x_test):
    #aux = resize(image,img_height))
    x_test[index] = aux / 255.0  # normalization
    index += 1




#### TRAINING STAGE ####

os.environ["KERAS_BACKEND"] = "tensorflow"
RANDOM_STATE = 42

def get_session(gpu_fraction=0.8):

    num_threads = os.environ.get('OMP_NUM_THREADS')
    gpu_options = tf.GPUOptions(per_process_gpu_memory_fraction=gpu_fraction)

    if num_threads:
        return tf.Session(config=tf.ConfigProto(
            gpu_options=gpu_options,intra_op_parallelism_threads=num_threads))
    else:
        return tf.Session(config=tf.ConfigProto(gpu_options=gpu_options))


#KTF.set_session(get_session())


def precision(y_true,y_pred):

    true_positives = K.sum(K.round(K.clip(y_true * y_pred,1)))
    predicted_positives = K.sum(K.round(K.clip(y_pred,1)))
    precision = true_positives / (predicted_positives + K.epsilon())
    return precision


def recall(y_true,1)))
    possible_positives = K.sum(K.round(K.clip(y_true,1)))
    recall = true_positives / (possible_positives + K.epsilon())
    return recall


def fbeta_score(y_true,y_pred,beta=1):

    if beta < 0:
        raise ValueError('The lowest choosable beta is zero (only precision).')

    # Set F-score as 0 if there are no true positives (sklearn-like).
    if K.sum(K.round(K.clip(y_true,1))) == 0:
        return 0.0

    p = precision(y_true,y_pred)
    r = recall(y_true,y_pred)
    bb = beta ** 2
    fbeta_score = (1 + bb) * (p * r) / (bb * p + r + K.epsilon())
    return fbeta_score


nb_classes = 4
final_model = []


# Option = InceptionV3
model = InceptionV3(weights="imagenet",include_top=False,input_shape=(img_width,3))
# Option = resnet
# model = resnet50(weights="imagenet",input_shape=(3,img_width,img_height))

# Creating new outputs for the model
x = model.output
x = Flatten()(x)
x = Dense(512,activation="relu")(x)
x = Dropout(0.5)(x)
x = Dense(512,activation="relu")(x)
x = Dropout(0.5)(x)
predictions = Dense(nb_classes,activation='softmax')(x)
#predictions = Dense(nb_classes,activation='sigmoid')(x)
final_model = Model(inputs=model.input,outputs=predictions)


# Metrics
learningRate = 0.001
optimizer = optimizers.SGD(learning_rate=learningRate,momentum=0.88,nesterov=True)

# Compiling the model...
final_model.compile(loss="categorical_crossentropy",optimizer=optimizer,metrics=["accuracy",fbeta_score])
final_model.summary()

#final_model.compile(loss = 'categorical_crossentropy',optimizer = 'adam',metrics = ['accuracy'])

#model.compile(loss = 'sparse_categorical_crossentropy',metrics = ['accuracy'])

#x_train = np.array(x_train)
#x_test = np.array(x_test)

x_train = np.asarray(x_train).astype(np.float32)
#x_test = np.array(x_test)
x_test = np.asarray(x_test).astype(np.float32)

# Defining targets...
y_train = np.concatenate([np.full((new_class_0_train.shape[0]),0),np.full((new_class_1_train.shape[0]),1),np.full((new_class_2_train.shape[0]),2),np.full((new_class_3_train.shape[0]),3)])

y_test = np.concatenate([np.full((new_class_0_test.shape[0]),np.full((new_class_1_test.shape[0]),np.full((new_class_2_test.shape[0]),np.full((new_class_3_test.shape[0]),3)])

y_train = to_categorical(y_train)
y_test =  to_categorical(y_test)

modelFilename = "/home/DEV/SKIN_SET_3000/model_inception.h5"

trainingFilename = "/home/DEV/SKIN_SET_3000/training.csv"
nb_train_samples = y_train.shape[0]
nb_test_samples = y_test.shape[0]
#epochs = 10000
epochs = 100
batch_size = 24
trainingPatience = 200
decayPatience = trainingPatience / 4

# Setting the data generator...
train_datagen = ImageDataGenerator(
    horizontal_flip=True,fill_mode="reflect",zoom_range=0.2
)

train_generator = train_datagen.flow(x_train,y_train,batch_size=batch_size)

# Saving the model
checkpoint = ModelCheckpoint(modelFilename,monitor='val_accuracy',verbose=1,save_best_only=True,save_weights_only=False,mode='auto',save_freq=1)

adaptativeLearningRate = ReduceLROnPlateau(monitor='val_accuracy',factor=0.5,patience=decayPatience,min_delta=0.0001,cooldown=0,min_lr=1e-8)

early = EarlyStopping(monitor='val_accuracy',min_delta=0,patience=trainingPatience,mode='auto')

csv_logger = CSVLogger(trainingFilename,separator=",",append=False)

# Callbacks
callbacks = [checkpoint,early,csv_logger,adaptativeLearningRate]

# Training of the model
final_model.fit(train_generator,steps_per_epoch=nb_train_samples / batch_size,epochs=epochs,shuffle=True,validation_data=(x_test,y_test),validation_steps=nb_test_samples / batch_size,callbacks=callbacks)

final_model.save('/home/DEV/SKIN_SET_3000/model_inception.h5')
#compile metrics

为了分割我的图像,首先我使用 SLIC 将我的输入图像转换为超像素

from skimage.segmentation import slic
from skimage.segmentation import mark_boundaries
from skimage.util import img_as_float
from skimage import io; io.use_plugin('matplotlib')
import cv2 as cv
from skimage.color import label2rgb

img_width,139

# load the model we saved
model = load_model('/home/DEV/SKIN_SET_3000/model_inception.h5',compile=False)

# Get test image ready
 img = skimage.img_as_float(skimage.io.imread('/home/DEV/SKIN_ULCER.jpg')).astype(np.float32)
plt.imshow(img)
test_image_slic = slic(img,n_segments=500,compactness=10.0)
test_image_slic_out = mark_boundaries(img,test_image_slic)
plt.imshow(test_image_slic_out)
#test_image=test_image/255
test_image_array = np.array(test_image_slic_out)
test_image_resize = cv2.resize(test_image_array,img_height))
test_image_reshape = test_image_resize.reshape(1,3)

我想检查我输入的每个超像素是否被标记为 4 个组织类中的目标类之一,并提取属于每个类的特征作为掩码并量化掩码的总表面积。 任何关于如何实施这种方法的建议将不胜感激。

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